4PHZ

Crystal structure of particulate methane monooxygenase from Methylocystis sp. ATCC 49242 (Rockwell)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	110
Detector technology	CCD
Collection date	2011-10-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.37765
Spacegroup name	P 21 21 21
Unit cell lengths	117.462, 184.531, 189.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.590
R-factor	0.2393
Rwork	0.237
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rfr
RMSD bond length	0.012
RMSD bond angle	1.666
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.590
Number of reflections	93278
<I/σ(I)>	18.9
Completeness [%]	72.6
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	10% PEG3000, 0.1 M sodium cacodylate trihydrate, pH 6.5, 0.2 M magnesium formate