4PHT

ATPase GspE in complex with the cytoplasmic domain of GspL from the Vibrio vulnificus type II Secretion system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	273
Detector technology	CCD
Collection date	2009-06-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	226.430, 133.900, 93.490
Unit cell angles	90.00, 91.41, 90.00

Refinement procedure

Resolution	43.220 - 2.830
R-factor	0.24923
Rwork	0.248
R-free	0.27977
RMSD bond length	0.008
RMSD bond angle	0.978
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.220
High resolution limit [Å]	2.690
Number of reflections	72192
<I/σ(I)>	10.41
Completeness [%]	95.2
Redundancy	1.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	PEG 3350