4PHB

Structure of the polysaccharide lyase-like protein Cthe_2159 from C. thermocellum, Gadolinium derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	68.810, 122.840, 34.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.510 - 2.178
R-factor	0.189038191589
Rwork	0.184
R-free	0.23407
RMSD bond length	0.008
RMSD bond angle	1.077
Data scaling software	SCALA ((phenix.refine: 1.9_1685))
Refinement software	PHENIX (1.9_1685)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.510
High resolution limit [Å]	2.178
Rmerge	0.134
Number of reflections	15967
<I/σ(I)>	7.98
Completeness [%]	99.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	19% PEG 8000, 0.1M MES pH 6.0, and 0.1M Calcium acetate