4PGY

Structure of human DNA polymerase beta complexed with a nicked DNA containing a GT at N-1 position and GC at N position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.440, 78.928, 54.348
Unit cell angles	90.00, 106.90, 90.00

Refinement procedure

Resolution	19.115 - 2.260
R-factor	0.2129
Rwork	0.210
R-free	0.26460
RMSD bond length	0.003
RMSD bond angle	0.635
Data reduction software	DENZO
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.260
Number of reflections	19762
<I/σ(I)>	20.52
Completeness [%]	94.8
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	14%?23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)