4PGW

Crystal structure of YetJ from Bacillus Subtilis at pH 6 by Pt-SAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-21
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.07
Spacegroup name	C 2 2 21
Unit cell lengths	47.640, 161.750, 123.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.768 - 3.600
R-factor	0.286
Rwork	0.284
R-free	0.33030
RMSD bond length	0.004
RMSD bond angle	0.794
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.600
Number of reflections	10695
<I/σ(I)>	14.01
Completeness [%]	99.7
Redundancy	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES, pH 6.0, 180 mM sodium acetate, 10-15% pentaerythritolethoxylate and 27% PEG 400