4PGS
Crystal structure of YetJ from Bacillus Subtilis at pH 6 by soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.833, 61.833, 276.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.456 - 2.500 |
| R-factor | 0.2372 |
| Rwork | 0.235 |
| R-free | 0.27370 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.851 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 11395 |
| <I/σ(I)> | 10.7 |
| Completeness [%] | 97.8 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 600, 100 mM CaCl2, 100 mM Tris-HCl, pH 8.0 |
