4PGL
Crystal structure of engineered D-tagatose 3-epimerase PcDTE-ILS6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.810, 47.440, 126.380 |
| Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
| Resolution | 36.850 - 2.100 |
| Rwork | 0.152 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qul |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.398 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.850 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.107 | 0.471 |
| Number of reflections | 69460 | |
| <I/σ(I)> | 12.1 | 3.7 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 0.1M MES, 10% w/v PEG 4000 |
