4PGA

GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-11
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	78.620, 135.800, 137.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.199
Rwork	0.199
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eca
RMSD bond length	0.010
RMSD bond angle	25.800 *
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.310	1.770
High resolution limit [Å]	1.710	1.710
Rmerge	0.075	0.261
Total number of observations	178566 *
Number of reflections	68971
<I/σ(I)>	19.9	2
Completeness [%]	86.0	62
Redundancy	2.6	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0 (M)