4PG1
Insights into Substrate and Metal Binding from the Crystal Structure of Cyanobacterial Aldehyde Deformylating Oxygenase with Substrate Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.122, 77.122, 116.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.580 - 1.700 |
| R-factor | 0.1808 |
| Rwork | 0.180 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tw3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.870 |
| Data reduction software | MD2 |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.600 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 39033 |
| <I/σ(I)> | 20 |
| Completeness [%] | 99.9 |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 35% PEG400, 0.1M Tris pH 8.5 |
