4PG0
Insights into Substrate and Metal Binding from the Crystal Structure of Cyanobacterial Aldehyde Deformylating Oxygenase with Substrate Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0781 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.354, 77.354, 115.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.440 - 1.900 |
R-factor | 0.1836 |
Rwork | 0.183 |
R-free | 0.20130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tw3 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.870 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.440 |
High resolution limit [Å] | 1.900 |
Number of reflections | 28419 |
<I/σ(I)> | 20 |
Completeness [%] | 99.9 |
Redundancy | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 35% PEG400, 0.1M Tris pH 8.5 |