4PFY
Crystal structure of mannohexaose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1223) from THERMOTOGA MARITIMA at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.638, 53.600, 106.269 |
| Unit cell angles | 90.00, 110.51, 90.00 |
Refinement procedure
| Resolution | 99.530 - 1.500 |
| R-factor | 0.16388 |
| Rwork | 0.163 |
| R-free | 0.18723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.199 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.530 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.604 | |
| Number of reflections | 163023 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MgNO3, 20-30% PEG 3350 |
