4PFT

Crystal structure of mannobiose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1223) from THERMOTOGA MARITIMA at 1.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-02-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	96.489, 53.540, 106.280
Unit cell angles	90.00, 110.47, 90.00

Refinement procedure

Resolution	46.066 - 1.747
R-factor	0.1545
Rwork	0.152
R-free	0.20370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vr5
RMSD bond length	0.008
RMSD bond angle	1.188
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.747	1.747
Rmerge		0.580
Number of reflections	103164
<I/σ(I)>	15.8	2.4
Completeness [%]	99.8	99.6
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MgNO3, 20-30% PEG 3350