4PFH
Crystal structure of engineered D-tagatose 3-epimerase PcDTE-IDF8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.460, 86.740, 61.820 |
| Unit cell angles | 90.00, 99.10, 90.00 |
Refinement procedure
| Resolution | 56.740 - 1.900 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.492 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.740 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.117 | 0.570 |
| Number of reflections | 46594 | |
| <I/σ(I)> | 9.6 | 4.6 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Na-HEPES, 25% w/v PEG 6000 |
