4PFB
Crystal structure of a TRAP periplasmic solute binding protein from Fusobacterium nucleatun (FN1258, TARGET EFI-510120) with bound SN-glycerol-3-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 113.574, 113.574, 79.937 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.154 - 2.700 |
R-factor | 0.1663 |
Rwork | 0.164 |
R-free | 0.20370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n6k |
RMSD bond length | 0.012 |
RMSD bond angle | 1.094 |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 113.574 | 46.295 | 2.850 |
High resolution limit [Å] | 2.700 | 8.540 | 2.700 |
Rmerge | 0.059 | 0.854 | |
Rmeas | 0.173 | ||
Rpim | 0.050 | 0.020 | 0.254 |
Total number of observations | 175821 | 5047 | 25682 |
Number of reflections | 14902 | ||
<I/σ(I)> | 10.4 | 18.2 | 3.1 |
Completeness [%] | 99.9 | 96.4 | 100 |
Redundancy | 11.8 | 9.6 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Protein (70.0 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 10 mM Glycerol 3-phosphate); Reservoir (0.2 M Zinc Acetate, 0.1 M Imidazole pH 6.5, 10 %(w/v) PEG 8000); Cryoprotection (80% Reservoir + 20% Glycerol |