4PEW

Structure of sacteLam55A from Streptomyces sp. SirexAA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	53.967, 92.567, 198.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.914 - 1.510
R-factor	0.1192
Rwork	0.117
R-free	0.16050
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.343
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.540
High resolution limit [Å]	1.510	4.100	1.510
Rmerge	0.058	0.037	0.306
Total number of observations	1641294
Number of reflections	142188
<I/σ(I)>	13
Completeness [%]	90.5	99.7	79.2
Redundancy	11.5	13.2	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	Protein Solution (20 mg/ml protein, 0.05 M NaCl, and 0.010 M MOPS pH 7) mixed in a 1:1 ratio with the Well Solution (23% PEG 3350, 200mM MgCl2, and 100mM BTP pH 7.0). Cryoprotected with 23% PEG 3350, 200mM MgCl2,100mM BTP pH 7.0, and 15% ethylene glycol