4PEV
Crystal structure of ABC transporter system solute-binding proteins from Aeropyrum pernix K1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.979, 109.601, 180.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.770 - 2.580 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.22850 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.580 | 7.050 | 2.600 |
| Rmerge | 0.195 | 0.039 | |
| Total number of observations | 239835 | ||
| Number of reflections | 44392 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 99.2 | 99.5 | 92.5 |
| Redundancy | 5.4 | 5.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 297 | 0.1M phosphate-citrate 1.6M Sodium phospahte/0.4M potassium phosphate |
