4PED
Mitochondrial ADCK3 employs an atypical protein kinase-like fold to enable coenzyme Q biosynthes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.671, 54.557, 45.009 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 36.634 - 1.640 |
| R-factor | 0.1672 |
| Rwork | 0.165 |
| R-free | 0.21100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.077 | 0.893 |
| Number of reflections | 69420 | |
| <I/σ(I)> | 23 | 1.5 |
| Completeness [%] | 97.0 | 94.8 |
| Redundancy | 7.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Seeded microbatch experiment, 5 mg/mL protein 12.5% PEG 3350, 50 mM HEPES pH 7.5, 5 mM MgCl2, 150 mM Am2SO4, 0.15 mM TCEP, 5 microliter sitting drop in bridge of VDX plate, equilibrated against 12.5% PEG 3350, 50 mM HEPES pH 7.5, 150 mM Am2SO4 |
