4PE6
Crystal structure of ABC transporter solute binding protein from Thermobispora bispora DSM 43833
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.724, 77.375, 79.472 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 31.660 - 1.860 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.21760 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.293 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 5.050 | 1.860 |
| Rmerge | 0.088 | 0.071 | 0.416 |
| Total number of observations | 193114 | ||
| Number of reflections | 53201 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 99.5 | 98 | 94 |
| Redundancy | 3.6 | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1M MES, 30% PEG4000 |
