4PDX
Crystal structure of Escherchia coli uncharacterized protein YjcS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.550, 76.182, 103.681 |
| Unit cell angles | 90.00, 115.26, 90.00 |
Refinement procedure
| Resolution | 45.118 - 1.750 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.21620 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.151 | 0.584 |
| Number of reflections | 131954 | |
| <I/σ(I)> | 36.8 | 5.2 |
| Completeness [%] | 98.8 | 97.4 |
| Redundancy | 7.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.8M (NH4)2SO4, 2% PEG 400, 0.1M HEPES (pH 7.5) |
