4PDT
Japanese Marasmius oreades lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SAGA-LS BEAMLINE BL07 |
| Synchrotron site | SAGA-LS |
| Beamline | BL07 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-21 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.395, 57.395, 57.944 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.380 - 1.400 |
| R-factor | 0.14751 |
| Rwork | 0.144 |
| R-free | 0.21027 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xd6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.819 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 14.380 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 22065 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 99.0 |
| Redundancy | 10.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.5 M Ammonium sulfate, 0.1 M Sodium citrate tribasic dihydrate, 1.0 M Lithium sulfate monohydrate, 5% 2-propanol |
