4PDL
Structure of K+ selective NaK mutant in caesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2009-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 4 |
| Unit cell lengths | 68.106, 68.106, 89.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.053 - 1.700 |
| R-factor | 0.2085 |
| Rwork | 0.207 |
| R-free | 0.23580 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 22437 |
| <I/σ(I)> | 49.8 |
| Completeness [%] | 99.9 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 65% MPD, 100mM CsCl, 100mM MES, 4mM DM |
