4PDE

Crystal structure of FdhD in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.984
Spacegroup name	P 61 2 2
Unit cell lengths	98.430, 98.430, 120.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.573 - 2.800
R-factor	0.1934
Rwork	0.190
R-free	0.27630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pw9
RMSD bond length	0.008
RMSD bond angle	1.178
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	85.000
High resolution limit [Å]	2.800
Number of reflections	8948
<I/σ(I)>	12.4
Completeness [%]	99.8
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.2M NaNO3, 2.2 M AmSO4