4PDB

CRYSTAL STRUCTURE OF BACILLUS ANTHRACIS RIBOSOMAL PROTEIN S8 IN COMPLEX WITH AN RNA APTAMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	103
Detector technology	CCD
Collection date	2009-08-31
Detector	NOIR-1
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	55.410, 59.270, 92.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.215 - 2.600
R-factor	0.1922
Rwork	0.189
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.260
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.092	0.643
Number of reflections	9796
<I/σ(I)>	11	2.7
Completeness [%]	99.9	99.9
Redundancy	7.08	6.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	283	0.3 M di-ammonium hydrogen citrate, 100 mM sodium chloride, 16% PEG 3350, 10 mM spermidine