4PCQ
Crystal Structure of MtbAldR (Rv2779c)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.739, 146.099, 49.961 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.190 - 2.950 |
| R-factor | 0.265 |
| Rwork | 0.263 |
| R-free | 0.29920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ivm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.953 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.194 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.430 | |
| Number of reflections | 17732 | |
| <I/σ(I)> | 25.6 | 3.4 |
| Completeness [%] | 99.5 | 93.1 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M trisodium citrate dihydrate, 20% PEG 3350 |
