4PCH
Structure of Human Polyomavirus 7 (HPyV7) VP1 pentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 209.670, 86.430, 84.210 |
| Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.17067 |
| Rwork | 0.169 |
| R-free | 0.19662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s7x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 161009 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 97.8 | 82.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100 mM Na-Malonate pH 4.5, 15% (w/v) PEG 3350 |
