4PC2
Elongation factor Tu:Ts complex with a bound GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.927, 108.510, 193.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.989 - 2.200 |
| R-factor | 0.1679 |
| Rwork | 0.165 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pc1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.044 | 0.252 |
| Number of reflections | 78193 | |
| <I/σ(I)> | 18.1 | 4.2 |
| Completeness [%] | 97.8 | 85.3 |
| Redundancy | 5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 100mM Hepes 73 mM Tris 17% PEG 6000 5% glycerol 150mM NaCl 1mM GDP 5mM EDTA |
