4PC2

Elongation factor Tu:Ts complex with a bound GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-22
Detector	MAR CCD 165 mm
Wavelength(s)	0.944
Spacegroup name	P 21 21 21
Unit cell lengths	73.927, 108.510, 193.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.989 - 2.200
R-factor	0.1679
Rwork	0.165
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pc1
RMSD bond length	0.010
RMSD bond angle	1.149
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.044	0.252
Number of reflections	78193
<I/σ(I)>	18.1	4.2
Completeness [%]	97.8	85.3
Redundancy	5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	100mM Hepes 73 mM Tris 17% PEG 6000 5% glycerol 150mM NaCl 1mM GDP 5mM EDTA