4PBZ
Structure of the human RbAp48-MTA1(670-695) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.800, 59.820, 68.070 |
Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.23080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xu7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.494 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.16) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.600 | 33.600 | 2.210 |
High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
Rmerge | 0.057 | 0.023 | 0.538 |
Rpim | 0.035 | 0.015 | 0.388 |
Total number of observations | 94637 | 949 | 4598 |
Number of reflections | 26112 | ||
<I/σ(I)> | 13 | 34.3 | 2.1 |
Completeness [%] | 97.3 | 90.9 | 80.8 |
Redundancy | 3.6 | 3.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v 2-Methyl-2,4-pentanediol (MPD), 0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M (RS)-1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, and 0.1 M MES/imidazole pH 6.5. |