4PBY

Structure of the human RbAp48-MTA1(656-686) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9809
Spacegroup name	P 1 21 1
Unit cell lengths	81.560, 59.480, 104.630
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.1809
Rwork	0.178
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RbAp48
RMSD bond length	0.012
RMSD bond angle	1.489
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.310	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.122	0.448
Number of reflections	35120
<I/σ(I)>	10.6	2.4
Completeness [%]	99.6	99.8
Redundancy	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	10% w/v PEG 20000, 20% v/v PEGMME 550, 0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M (RS)-1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, and 0.1 M MES/imidazole pH 6.5