4PBX
Crystal structure of the six N-terminal domains of human receptor protein tyrosine phosphatase sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 198.780, 198.780, 132.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 172.150 - 3.150 |
| R-factor | 0.23562 |
| Rwork | 0.234 |
| R-free | 0.26497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd3 2yd4 2yd9 2dju |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.400 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.078 | 0.991 |
| Number of reflections | 25619 | |
| <I/σ(I)> | 17.8 | 2.1 |
| Completeness [%] | 95.6 | 96.1 |
| Redundancy | 7.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.5 | 10 % PEG 400, 0.01 M magnesium chloride, 0.1 M potassium chloride, 0.05 M MES pH 6.0 |
