4PBO
Crystal structure of zebrafish short-chain pentraxin protein without calcium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98048 |
| Spacegroup name | H 3 |
| Unit cell lengths | 114.745, 114.745, 61.023 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.000 - 1.701 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.18600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.033 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.274 | |
| Number of reflections | 32265 | |
| <I/σ(I)> | 31.4 | 7.111 |
| Completeness [%] | 98.0 | 92 |
| Redundancy | 10.8 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.085M HEPES, 1.7% v/v polyethylene glycol 400, 1.7M ammonium sulphate, 15% v/v glycerol |
