4PBG
6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-01 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.700, 174.300, 60.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.000 * |
| Data reduction software | XDS |
| Data scaling software | BIOMOL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 * |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.090 * |
| Number of reflections | 32734 * |
| Completeness [%] | 84.0 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | PEG-4000, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | drop | o-Nitrophenyl-beta-D-galactopyranoside-6-phosphate | 40 (mM) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | PEG4000 | 28 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 8 | 1 | reservoir | 0.02 (%) |
