4PBB

The 5-Hydroxymethylcytosine-Specific Restriction Enzyme AbaSI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	67.428, 92.352, 180.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.206 - 3.460
R-factor	0.2426
Rwork	0.240
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	low resolution Se-SAD structures
RMSD bond length	0.003
RMSD bond angle	0.830
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.210	3.610
High resolution limit [Å]	3.450	3.450
Rmerge	0.166
Number of reflections	15301
<I/σ(I)>	9.8
Completeness [%]	100.0	100
Redundancy	4.8	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	ammonium sulfate, HEPES