4PBA
The 5-Hydroxymethylcytosine-Specific Restriction Enzyme AbaSI in a Complex with Substrate-like DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.673, 147.133, 103.485 |
| Unit cell angles | 90.00, 94.34, 90.00 |
Refinement procedure
| Resolution | 35.090 - 3.301 |
| R-factor | 0.2426 |
| Rwork | 0.240 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | low resolution SAD determined structure |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.664 |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.090 | 3.410 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.211 | |
| Number of reflections | 24388 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 289 | PEG 3350 ammonium tartrate BisTris buffer calcium acetate |
