4PAY

Crystal structure of an N-terminal fragment of the Legionella pneumophila effector protein SidC.

Replaces:  4OM6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	105.460, 107.584, 177.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.009 - 2.770
R-factor	0.2022
Rwork	0.199
R-free	0.24640
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.989
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	92.009
High resolution limit [Å]	2.770
Number of reflections	52258
<I/σ(I)>	17.8
Completeness [%]	99.8
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277.15	8% PEG 8000, 0.2M barium chloride, 0.1M Hepes, pH 7.4