4PAR
The 5-Hydroxymethylcytosine-Specific Restriction Enzyme AbaSI in a Complex with Product-like DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.826, 144.704, 105.033 |
| Unit cell angles | 90.00, 94.57, 90.00 |
Refinement procedure
| Resolution | 34.900 - 2.890 |
| R-factor | 0.1927 |
| Rwork | 0.190 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model from 2 SAD expts |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.894 |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 2.990 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.089 | 0.789 |
| Number of reflections | 35953 | |
| <I/σ(I)> | 13.4 | 1.4 |
| Completeness [%] | 98.6 | 89.3 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 289 | PEG 3350, ammonium tartrate, HEPES |
