4PA1
Crystal Structure of Catalytic Core domain of FIV Integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-03 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.924, 52.924, 193.569 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.830 - 1.840 |
| R-factor | 0.17375 |
| Rwork | 0.171 |
| R-free | 0.22155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9ka |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.812 |
| Data scaling software | HKL-3000 (5.8.0049) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.830 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.844 |
| Rmerge | 0.100 | 0.354 |
| Number of reflections | 13341 | |
| <I/σ(I)> | 29.66 | 6.74 |
| Completeness [%] | 90.6 | 88.21 |
| Redundancy | 15.2 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 200 mM MgCl2, 100 mM TrisHCl pH 8.5 |
