4P9X
Structure of ConA/Rh3Glu complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.173, 116.028, 84.255 |
| Unit cell angles | 90.00, 95.98, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.060 |
| R-factor | 0.2465 |
| Rwork | 0.245 |
| R-free | 0.29530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.111 |
| Data scaling software | XDS |
| Phasing software | MOLREP (11.0.02) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.400 |
| High resolution limit [Å] | 2.060 |
| Number of reflections | 98988 |
| <I/σ(I)> | 17.63 |
| Completeness [%] | 99.7 |
| Redundancy | 3.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.2 | 298 | buffer: 20 mM HEPES, 5 mM of CaCl2, and 5 mM of MnCl2; pH = 7.2. The crystal was obtained by diffusion method in glass tube sequentially put with ConA solution, pure buffer, and the ligand solution. |
