4P9W
Structure of ConA/Rh3Man
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.000, 116.030, 84.100 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 39.400 - 2.110 |
| R-factor | 0.1737 |
| Rwork | 0.173 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.045 |
| Data scaling software | XDS |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.400 |
| High resolution limit [Å] | 2.110 |
| Number of reflections | 88321 |
| <I/σ(I)> | 26.99 |
| Completeness [%] | 95.7 |
| Redundancy | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.2 | 298 | buffer: 20 mM HEPES, 5 mM of CaCl2, and 5 mM of MnCl2; pH = 7.2. The crystal was obtained by diffusion method in glass tube sequentially put with ConA solution, pure buffer, and the ligand solution. |
