4P9R

Speciation of a group I intron into a lariat capping ribozyme (Heavy atom derivative)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.10530; 1.10564; 1.09553; 1.11525
Spacegroup name	P 21 21 21
Unit cell lengths	59.440, 86.260, 109.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.945 - 2.703
R-factor	0.2121
Rwork	0.210
R-free	0.25640
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.504
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.900
High resolution limit [Å]	2.700
Number of reflections	29825
<I/σ(I)>	12.38
Completeness [%]	99.9
Redundancy	6.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	(i) 0.2 Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% w/v PEG 3,350 or (ii) 0.2 Sodium chloride, 0.1 M HEPES pH 7.5, 25% w/v PEG 3,350