4P9O
Complex of Voltage-gated ion channel in a the presence of channel blocking compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.420, 328.370, 80.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.910 - 2.890 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.25070 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.240 | 3.070 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.191 | 0.714 |
| Number of reflections | 24368 | |
| <I/σ(I)> | 12.7 | 3.6 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 13.5 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M sodium citrate, 0.1 M Tris, 34% PEG |
