4P9L

Crystal Structure of mouse Ryanodine Receptor 2 SPRY2 Domain (1080-1253) disease mutant A1107M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 2 21 21
Unit cell lengths	36.738, 66.989, 67.232
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.450 - 1.436
R-factor	0.1536
Rwork	0.151
R-free	0.18420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.142
Refinement software	PHENIX ((phenix.refine: dev_1637))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.450
High resolution limit [Å]	1.436
Number of reflections	28739
<I/σ(I)>	24.3
Completeness [%]	92.6
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	sodium formate, peg 3350