4P9K

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM VERMINEPHROBACTER EISENIAE EF01-2 (Veis_3954, TARGET EFI-510324) A NEPHRIDIAL SYMBIONT OF THE EARTHWORM EISENIA FOETIDA, BOUND TO D-ERYTHRONATE WITH RESIDUAL DENSITY SUGGESTIVE OF SUPERPOSITION WITH COPURIFIED ALTERNATIVE LIGAND.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-21
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	76.290, 76.290, 110.813
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.535 - 1.400
R-factor	0.1489
Rwork	0.148
R-free	0.16800
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.202
Data reduction software	MOSFLM (0.1.27)
Data scaling software	Aimless
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	110.810	110.810	1.420
High resolution limit [Å]	1.400	7.670	1.400
Rmerge	0.127	0.070	2.287
Rpim	0.032	0.019	0.579
Total number of observations	1228244	7163	59168
Number of reflections	142034
<I/σ(I)>	16	46.1	1.7
Completeness [%]	100.0	99.2	100
Redundancy	16.6	13.5	16.4
CC(1/2)	0.999	0.998	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Protein (42.92 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 1 mM D-Erythronate); Reservoir ( 0.1 M Tris pH 8.5 2.4 M di-Ammonium Phosphate ); Cryoprotection (Reservoir + 20% glycerol