4P9I

Crystal Structure of mouse Ryanodine Receptor 2 SPRY2 Domain (1080-1253)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	36.290, 66.230, 66.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.095 - 1.340
R-factor	0.1389
Rwork	0.138
R-free	0.16310
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1637))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.095
High resolution limit [Å]	1.340
Number of reflections	33935
<I/σ(I)>	17.6
Completeness [%]	91.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	sodium formate, peg 3350