4P9D
Crystal structure of dCMP deaminase from the cyanophage S-TIM5 in complex with dTMP and dTTP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.900, 79.020, 126.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.022 - 2.900 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.020 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.089 | 0.349 |
| Number of reflections | 17452 | |
| <I/σ(I)> | 11.7 | 4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Potassium formate, 20% (w/v) PEG 3350, 5mM Magnesium chloride and 5mM dTTP |
