4P99
Ca2+-stabilized adhesin helps an Antarctic bacterium reach out and bind ice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-01-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.464, 47.469, 191.161 |
| Unit cell angles | 90.04, 90.01, 90.02 |
Refinement procedure
| Resolution | 47.469 - 1.800 |
| R-factor | 0.2223 |
| Rwork | 0.221 |
| R-free | 0.25710 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.443 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 148953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 0.2 M calcium chloride, 0.1 M MES (pH 6) and 20% (w/v) PEG 6000 |
