4P98

ABC transporter system solute-bindng protein from Conexibacter woesei DSM 14684

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91975
Spacegroup name	P 41 21 2
Unit cell lengths	79.091, 79.091, 139.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.680 - 1.900
R-factor	0.1592
Rwork	0.157
R-free	0.20310
RMSD bond length	0.007
RMSD bond angle	1.162
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.071	0.036	0.820
Total number of observations	495004
Number of reflections	35740
<I/σ(I)>	11.8
Completeness [%]	99.9	97.6	100
Redundancy	13.9	12	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	2.0 M Ammonium Sulfate, 0.1 M Tris-Cl