4P8Z

Speciation of a group I intron into a lariat capping ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	52.560, 88.860, 105.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.550 - 3.850
R-factor	0.2481
Rwork	0.245
R-free	0.29770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4P95
RMSD bond length	0.001
RMSD bond angle	0.842
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.550
High resolution limit [Å]	3.850
Number of reflections	5021
<I/σ(I)>	9.63
Completeness [%]	99.6
Redundancy	6.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine ED-2001 pH 7.0