4P8S

Crystal structure of Nogo-receptor-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	56.822, 56.517, 128.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
R-factor	0.17317
Rwork	0.171
R-free	0.20472
RMSD bond length	0.031
RMSD bond angle	2.413
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge		0.412
Number of reflections	38016
<I/σ(I)>	18.3	2.9
Completeness [%]	97.3	76.9
Redundancy	1.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M hepes, 10% isopropanol, 15% PEG 10K