4P7Y
L-methionine gamma-lyase from Citrobacter freundii with Y58F substitution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.900 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.546, 124.531, 129.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.960 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rfv |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.961 |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.330 | |
| Number of reflections | 31059 | |
| <I/σ(I)> | 7.3 | 2.32 |
| Completeness [%] | 96.6 | 84.6 |
| Redundancy | 6.22 | 4.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 5% PEG2000 MME, 50 MM TRIS-HCL, 0.2 MM PLP, 0.25% DTT, pH 8.5 |
