4P7W
L-proline-bound L-proline cis-4-hydroxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-05-22 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 55.390, 73.615, 95.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.800 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.468 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | MOLREP (11.1.03) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.535 |
| Number of reflections | 36588 | |
| <I/σ(I)> | 6.9 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | Bis-Tris propane, Sodium malonate, PEG 3350 |
